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anti-hmgb1 blocking antibody  (Feinstein Institute)

 
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    Feinstein Institute anti-hmgb1 blocking antibody
    (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 <t>(HMGB1)</t> levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.
    Anti Hmgb1 Blocking Antibody, supplied by Feinstein Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-hmgb1 blocking antibody/product/Feinstein Institute
    Average 90 stars, based on 1 article reviews
    anti-hmgb1 blocking antibody - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study"

    Article Title: Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study

    Journal: PLoS Medicine

    doi: 10.1371/journal.pmed.1002522

    (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 (HMGB1) levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.
    Figure Legend Snippet: (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 (HMGB1) levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.

    Techniques Used: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Blocking Assay, Control, Injection

    We hypothesize that when resuscitation with stored red blood cells (RBCs) leads to increased circulating heme that is in excess of hemopexin, 2 parallel pathways are activated involving (i) release of HMGB1 and subsequent decreased immune-cell-dependent bacterial clearance and (ii) increased endothelial permeability. These combine to promote acute lung injury and increase sensitivity to lung infection. We also underscore the potential that heme-dependent effects synergize with other components of stored RBCs, including hemoglobin, non-transferrin-bound iron (NTBI), and microvesicles, to mediate adverse outcomes.
    Figure Legend Snippet: We hypothesize that when resuscitation with stored red blood cells (RBCs) leads to increased circulating heme that is in excess of hemopexin, 2 parallel pathways are activated involving (i) release of HMGB1 and subsequent decreased immune-cell-dependent bacterial clearance and (ii) increased endothelial permeability. These combine to promote acute lung injury and increase sensitivity to lung infection. We also underscore the potential that heme-dependent effects synergize with other components of stored RBCs, including hemoglobin, non-transferrin-bound iron (NTBI), and microvesicles, to mediate adverse outcomes.

    Techniques Used: Permeability, Infection



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    (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 <t>(HMGB1)</t> levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.
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    (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 <t>(HMGB1)</t> levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.
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    (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 <t>(HMGB1)</t> levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.
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    Image Search Results


    (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 (HMGB1) levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.

    Journal: PLoS Medicine

    Article Title: Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study

    doi: 10.1371/journal.pmed.1002522

    Figure Lengend Snippet: (A) C57BL/6 wild-type mice underwent trauma hemorrhage (TH) and were randomly resuscitated with either fresh ( n = 3) or stored ( n = 3) red blood cells (RBCs) with plasma (1:1). Blood was drawn 6 h after resuscitation, and high mobility group box 1 (HMGB1) levels were measured by ELISA. Data are mean ± SEM; each symbol represents an individual mouse. * p = 0.018 compared to mice resuscitated with fresh blood by unpaired t test. (B) Rat microvascular endothelial cell (RMVEC) monolayers were exposed to vehicle (0.01 M NaOH, n = 5) or hemin (10 μM, n = 5) for 6 h, and supernatants were analyzed by ELISA for extracellular HMGB1. Data show mean ± SEM. * p = 0.003 compared to vehicle by unpaired t test. (C) For phagocytosis assays, MH-S cells were pretreated with anti-HMGB1 blocking antibody or isotype control antibody prior to exposure to hemin and P . aeruginosa K-strain (PAK). Colonies were counted, and phagocytosis expressed as percent of control, with 100 percent being represented by colony number in plates in vehicle-treated cells. Data were collected from 3 independent experiments with 3 replicates in each experiment. Data are mean ± SEM; each symbol represents an individual experimental mean. * p = 0.023 relative to control and ** p = 0.045 relative to heme + isotype antibody by 1-way repeated measures ANOVA with Tukey post-test. (D) C57BL/6 wild-type mice underwent TH and resuscitation with stored RBCs with plasma (1:1). Forty-eight hours later, these mice were instilled with PAK, and survival monitored. Before instillation of PAK, mice were injected peritoneally with either anti-HMGB1 blocking antibody ( n = 6) or isotype control antibody ( n = 4). * p = 0.001 compared to isotype control by log-rank test.

    Article Snippet: Anti-HMGB1 blocking antibody was a kind gift from Kevin Tracey (Feinstein Institute for Medical Research, New York, NY).

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Blocking Assay, Control, Injection

    We hypothesize that when resuscitation with stored red blood cells (RBCs) leads to increased circulating heme that is in excess of hemopexin, 2 parallel pathways are activated involving (i) release of HMGB1 and subsequent decreased immune-cell-dependent bacterial clearance and (ii) increased endothelial permeability. These combine to promote acute lung injury and increase sensitivity to lung infection. We also underscore the potential that heme-dependent effects synergize with other components of stored RBCs, including hemoglobin, non-transferrin-bound iron (NTBI), and microvesicles, to mediate adverse outcomes.

    Journal: PLoS Medicine

    Article Title: Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study

    doi: 10.1371/journal.pmed.1002522

    Figure Lengend Snippet: We hypothesize that when resuscitation with stored red blood cells (RBCs) leads to increased circulating heme that is in excess of hemopexin, 2 parallel pathways are activated involving (i) release of HMGB1 and subsequent decreased immune-cell-dependent bacterial clearance and (ii) increased endothelial permeability. These combine to promote acute lung injury and increase sensitivity to lung infection. We also underscore the potential that heme-dependent effects synergize with other components of stored RBCs, including hemoglobin, non-transferrin-bound iron (NTBI), and microvesicles, to mediate adverse outcomes.

    Article Snippet: Anti-HMGB1 blocking antibody was a kind gift from Kevin Tracey (Feinstein Institute for Medical Research, New York, NY).

    Techniques: Permeability, Infection